Faculty : Mathematics And Science
Study Program : Biology Education
Subject : Biotechnology
Credit : Theory 2 unit of semester credit
Semester : VI
Prerequisite Subject : Biology Cell and Moleculer, Microbiology, Genetics
Lecturer : Evy Yulianti, M.Sc., Paramita Cahyaningrum K., M.Sc., Lili Sugiyarto, M.Si.
I. Subject Description
Biotechnology study the technologies that involve the use of living organisms or products from living organisms in order to benefit humans usually for medical, agricultural and industrial applicationII. Standard of Competence
The main goal of this subject for students is to gain an understanding of the basic technique in biotechnology and the medical, agricultural and industrial applicationIII. Lesson Plans
Meeting
|
Basic of Competence
|
Topics
|
Strategy
|
References
|
Character
|
1st
|
Describe what
biotechnology is and the subject supporting biotechnology, describe the history
of biotechnology
|
Introduction
- Meaning of biotechnology
- Subject supporting biotechnology
- History of biotechnology
|
Lecturer,
presentation and discussion
|
Appreciating
diversity/appre ciation of diversity, Curious/curios ity, Passionate about learning/passi on for learning
| |
2nd
|
Describe the PCR
reaction mixture, condition and the use of PCR
|
Basic Technique in
molecular Biology: PCR
- PCR reaction mixture
- PCR Conditions
- PCR-RFLP
|
Lecturer,
presentation and discussion
|
Appreciating
diversity/appre ciation of diversity, Curious/curios ity, Passionate about learning/passi on for learning
|
- Principles and
Medical Applications of the Polymerase Chain Reaction
| |||||
3rd
|
Describe how DNA
being manipulated, explain kind of restriction enzyme and how they work, explain how to make recombinant DNA and the finction of recombinant DNA
|
Basic Technique in
molecular Biology: DNA recombinant technology
- The Manipulation of Nucleic Acids: Basic Tools and Techniques
- Restriction
Enzymes
- Use of restriction enzymes
- Recombinant DNA
- Transformation of
Bacteria
- Constructing DNA
libraries
|
Lecturer,
presentation and discussion
|
Curious/curios
ity, Passionate about learning/passi on for learning
| |
4th
|
Explain
biotechnology application in human gene therapy, IVF and DNA fingerprinting
|
Biotechnology
application : in health and forensic
- Human Gene
Therapy
- The technique of
IVF
- DNA
fingerprinting
|
Lecturer,
presentation and discussion
|
Curious/curios
ity, Passionate about learning/passi on for learning
| |
5th
|
Explain
biotechnology application in cloning
|
Biotechnology
application : in livestock
- Cloning
|
Lecturer,
presentation and discussion
|
Curious/curios
ity, Passionate about learning/passi on for learning
| |
6th
|
Describe the history
of plant biotechnology, methods used in plant biotechnology (tissue culture and
transgenic)
|
Methods of Plant
Biotechnology
|
Lecture and discussion
|
D and E
(see list of references below)
| |
7th
|
Describe the method
of obtaining
|
Plant Transgenesis
|
Lecture and
|
D and E
|
transgenic plants and
the use of molecular markers
|
discussion
|
(see list of
references below)
| |||
8th
|
Midterm I
| ||||
9th
|
Describe the methods
used for several examples of the application of plant biotechnology (vaccine for plants, genetic pesticides, herbicide resistance, enhanced nutrition, pharmacology, fuels)
|
Application of Plant
Biotechnology
|
Lecture and discussion
|
D and E
(see list of references below)
| |
10th
|
Describe and analyze
the negative and positive effects of biotechnology on the development of new plant varieties.
|
Health and environmental concerns
|
Lecture and discussion
|
D and E
(see list of references below)
| |
11th
|
Presentation
| ||||
12 th
|
Microbial
biotechnology (scope, technique)
Microbial diversity Introduction of DNA into yeast cell
|
Lecture and
discussion
| |||
13 th
|
Industrial application
Fermentation process and fermenters Production of antibiotics
Goals of antibiotic research
|
Lecture and
discussion
| |||
14 th
|
Environtmental
application
The process of biodegradation Biodegradation of organic pollutants Bioremediation
|
Lecture and
discussion
| |||
15 th
|
Human isulin gene
Production of microbial enzyme Application of microbia enzyme
|
Lecture and
discussion
| |||
16 th
|
Presentation
|
IV. Reference
Compulsory reading :
A. Agrawal, S. 2008. Techniques in Molecular Biology. International Book
Distributing Co.
B. Popping B, Diaz-Amigo C, Hoenicke K. 2010. Molecular Biological And Immunological Techniques And Applications For Food Chemists.. John Wiley &Sons, Inc.
C. Walker, J. M., Rapley R. 2008 Molecular Biomethods Handbook. Humana Press, a part of Springer Science
D. Mantell, S.H., J.A.Matthews, and R.A.McKee. 1985. Principles of Plant
Biotechnology. Blackwell Scientific Publications. London. UK. 258p
E. Renneberg, R. 2006. Biotechnology for Beginners. (Ed. Arnold.L.Demain).
Elsevier Inc. Muenchen.Germany.pp. 171-202.
F. Syukur, M., S.Sujiprihati, dan R.Yunianti.2012. Teknik Pemuliaan Tanaman.
Penebar Swadaya. Hal 173-184
G. Thieman, W.J., and M.A.Palladino. 2013. Introduction to Biotechnology. Pearson Education, Inc. USA. Pp.158-174
V. Evaluation
No
|
Component of evaluation
|
Score ( % )
|
1
|
Attendance
|
10
|
2
|
Participation
|
20
|
3
|
Assignment
|
20
|
4
|
Midterm
|
20
|
5
|
Final Examination
|
30
|
Total
|
100
|
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